Sequencing:Article Title:
Article Snippet: .. Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 .. Reagent Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12364 * https://www.nature.com/articles/s41467-020-19343-2 ** https://www.mdpi.com/1422-0067/21/24/9666 *** https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.13037 **** https://www.nature.com/articles/ncomms5848#Sec2 ***** https://www.nature.com/articles/s41477-022-01303-x ******
Article Title:
Article Snippet: Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 .. Reagent Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12364 * https://www.nature.com/articles/s41467-020-19343-2 ** https://www.mdpi.com/1422-0067/21/24/9666 *** https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.13037 **** https://www.nature.com/articles/ncomms5848#Sec2 ***** https://www.nature.com/articles/s41477-022-01303-x ****** ..
Modification:Article Title:
Article Snippet: .. Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 .. Reagent Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12364 * https://www.nature.com/articles/s41467-020-19343-2 ** https://www.mdpi.com/1422-0067/21/24/9666 *** https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.13037 **** https://www.nature.com/articles/ncomms5848#Sec2 ***** https://www.nature.com/articles/s41477-022-01303-x ******
Article Title:
Article Snippet: Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 .. Reagent Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12364 * https://www.nature.com/articles/s41467-020-19343-2 ** https://www.mdpi.com/1422-0067/21/24/9666 *** https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.13037 **** https://www.nature.com/articles/ncomms5848#Sec2 ***** https://www.nature.com/articles/s41477-022-01303-x ****** ..
Plasmid Preparation:Article Title:
Article Snippet: .. Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 .. Reagent Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12364 * https://www.nature.com/articles/s41467-020-19343-2 ** https://www.mdpi.com/1422-0067/21/24/9666 *** https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.13037 **** https://www.nature.com/articles/ncomms5848#Sec2 ***** https://www.nature.com/articles/s41477-022-01303-x ******
Article Title:
Article Snippet: Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 .. Reagent Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12364 * https://www.nature.com/articles/s41467-020-19343-2 ** https://www.mdpi.com/1422-0067/21/24/9666 *** https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.13037 **** https://www.nature.com/articles/ncomms5848#Sec2 ***** https://www.nature.com/articles/s41477-022-01303-x ****** ..
Magnetic Beads:Article Title:
Article Snippet: .. Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 .. Reagent Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12364 * https://www.nature.com/articles/s41467-020-19343-2 ** https://www.mdpi.com/1422-0067/21/24/9666 *** https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.13037 **** https://www.nature.com/articles/ncomms5848#Sec2 ***** https://www.nature.com/articles/s41477-022-01303-x ******
Article Title:
Article Snippet: Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 .. Reagent Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12364 * https://www.nature.com/articles/s41467-020-19343-2 ** https://www.mdpi.com/1422-0067/21/24/9666 *** https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.13037 **** https://www.nature.com/articles/ncomms5848#Sec2 ***** https://www.nature.com/articles/s41477-022-01303-x ****** ..
Reverse Transcription:Article Title:
Article Snippet: .. Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 .. Reagent Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12364 * https://www.nature.com/articles/s41467-020-19343-2 ** https://www.mdpi.com/1422-0067/21/24/9666 *** https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.13037 **** https://www.nature.com/articles/ncomms5848#Sec2 ***** https://www.nature.com/articles/s41477-022-01303-x ******
Article Title:
Article Snippet: Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 .. Reagent Company catalog number GFP-Trap® Magnetic Agarose ChromoTek gtma-20 MagRackTM 6, Cytiva GE Healthcare 89129-096 Protein sample Buffer, Laemmli 2× Concentrate Merck S3401-10VL TEAB buffer Merck 15715-58-9 Sequencing Grade Modified Trypsin Promega V5111 pMAL-c2X® vector Addgene 75286 pTrcHis-TRF2 vector Addgene 50488 SifI restriction enzyme NEB R0123S DraIII restriction enzyme NEB R3510S pDONR221® vector Invitrogen 12536017 pGDAT7®and pGBKT7® vectors Clontech 630489 35S::GVG:Nos cassette Synbio TSL pICSL11041 Ni-NTA Agarose QIAGEN 30210 UDP-GlcNAc Merck U4375 GDP-fucose Merck G4401 PierceTM Anti-HA Magnetic Beads Thermo Scientific 88836 Erythrosin B Sigma-Aldrich 198269 potassium iodide Sigma-Aldrich 7681-11-0 PierceTM D-Luciferin Thermo Scientific 88291 Yeast Synthetic Drop-out medium (-L -W) Sigma-Aldrich Y0750-20G Yeast Synthetic Drop-out medium (-L -W -H -A) Sigma-Aldrich Y2021-20G 3-AT (3-Amino-1,2,4-triazole) Merck A8056 Dexamethasone Merck D1756 DMSO Merck 472301 6-benzylaminoadenine Merck B3408 RNeasy Plant Mini Kit QIAGEN 74904 M-MLV Reverse Transcriptase Promega M1701 qPCRBIO SyGreen Blue Mix PCRbio PB20.15-01 GFP Monoclonal Antibody (*) Thermo Scientific GF28R Biotin anti-RFP antibody (**) abcam ab34771 anti-mouse antibody (m-IgGκ BP-HRP) (***) Santa Cruz sc-516102 Goat Anti-Rabbit IgG H&L (HRP) (****) abcam ab205718 Anti-HA-Biotin (*****) Merck 3F10 Monoclonal ANTI-FLAG® M2 antibody (******) Merck F3165 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12364 * https://www.nature.com/articles/s41467-020-19343-2 ** https://www.mdpi.com/1422-0067/21/24/9666 *** https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.13037 **** https://www.nature.com/articles/ncomms5848#Sec2 ***** https://www.nature.com/articles/s41477-022-01303-x ****** ..
|
TaKaRa is a verified supplier 
TaKaRa manufactures this product 